Biochemistry

Content:

Part 1:

• Chemical bonding in simple organic and inorganic molecules, Lewis theory, Valence bond theory and molecular orbital theory
• Alkanes, achenes, alkynes (structure and reactivity, nomenclature)
• Alcohol, Ether, esters, aldehydes, ketones (structure and reactivity, nomenclature)
• Isomerism: stereo isomers, configurational, conformational isomers, enantiomers, diasteroisomers
• Prototypical reactions of organic molecules: nucleophilic substitutions, addition and elimination reactions, competition among the possible paths
• Conjugated polyenes and benzene, aromaticity
• Forces determining interactions among molecules: hydrogen bonds, hydrophobic, dispersion, cation pi interactions
• Acid base reactions and factors affecting acidity/basicity in organic molecules (inductive effect and mesomeric effect)
• Acid base catalysis
• Michaelis Menten equation

Part 2:

• Introduction to the cell
• Amino acids, polypeptides, post transcriptional modification of amino acids
• Protein structures, primary, secondary and tertiary structures, alpha helices, beta sheets, Ramachandran plot
• Globular, membrane and disordered proteins, Protein folding
• Protease enzymes: serine
• protease, metallo protease, aspartic protease, thermolysin.
• Serine protease and serine and metallo beta lactamase, lysozyme,
• Enzyme processing Phosphate groups: P-type ATPases, kinases, phosphatases
• Rotational catalysis: ATP synthase
• Hemoglobin, allostery in hemoglobin
• DNA structure and function, replication, proofreading, repair enzymes
• RNA structure, transcription, splicing translation, spliceosome, ribosome, ribozymes.
• Membranes, lipids, transporters, channels, ligand gated channels, GPCR